The study of single cell -omics has generated enormous interest in the scientific field over the last few years. The analysis of complex biological systems at the single cell level provides insights into fundamental biological phenomena that were either hidden or only partly discernible using previous approaches that average data across a population of cells.
Until recently, single cell analysis of proteins has been limited to immunofluorescent techniques and has remained out of reach of broad discovery methods such as mass spectrometry.
Nikolai Slavov, an Assistant Professor at Northeastern University, has developed a TMT-based approach to Single Cell Proteomics by Mass Spectrometry (SCoPE-MS) that is made possible using IonOpticks Aurora series columns. In his most recent work, they report the SCoPE2 methodology that substantially increases the capabilities of the original SCoPE method, reducing cost and analysis time.
In this recent genome webinar, Harrison Specht from the Slavov Lab describes how SCoPE2 lowers cost and hands-on time for proteomic analysis of single cells. Hear how this was achieved using only commercially available equipment and reagents with IonOpticks columns here.
Read the full paper
Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2.
Genome Biol 22, 50 (2021). https://doi.org/10.1186/s13059-021-02267-5
Specht H, Emmott E, Petelski AA, Huffman RG, Perlman DH, Serra M, Kharchenko P, Koller A, Slavov N.
Commentary by Andrew Webb, PhD.
About the author
Andrew has over 15 years’ experience in the field of chromatography and mass spectrometry. He is the lead innovator and inventor at IonOpticks, working closely with the team to test, refine and develop cutting edge techniques to support higher quality outputs and analytics from MS instruments. Andrew is also the Lab Head of the Walter and Eliza Hall Institute of Medical Research’s Proteomics Research Laboratory.