A new sensitive and accurate method enables endogenous phosphotyrosine mapping.

Phosphotyrosine (pTyr) plays a central role in many cell-to-cell communication pathways, including those that regulate proliferation, differentiation, adhesion, hormone responses, and immune defense. These pathways are commonly dysregulated in cancer and have provided attractive targets for therapeutic intervention.

Historically, measurement of global changes of pTyr has predominantly been limited to model systems, requiring large amounts of input material due the low abundant nature of these modifications. In this recent BioRxiv publication, Stopfer and colleagues demonstrate a workflow, termed “SureQuant pTyr”, that can reliably and accurately quantify hundreds of endogenous pTyr targets.

The plug and play nature of IonOpticks Aurora Series columns helped in facilitating the turn-key nature of this approach that opens the door to rapid and accurate profiling in clinical research settings.

Read the full paper
High-density, targeted monitoring of tyrosine phosphorylation reveals activated signaling networks in human tumors.
BioRxiv
. 2020 Jun 2. doi: https://doi.org/10.1101/2020.06.01.127787
Stopfer LE, Flower CT, Gajadhar AS, Patel B, Gallien S, Lopez-Ferrer D, White FM.

Commentary by Andrew Webb, PhD.

About the author
Andrew has over 15 years’ experience in the field of chromatography and mass spectrometry. He is the lead innovator and inventor at IonOpticks, working closely with the team to test, refine and develop cutting edge techniques to support higher quality outputs and analytics from MS instruments. Andrew is also the Lab Head of the Walter and Eliza Hall Institute of Medical Research’s Proteomics Research Laboratory.