An inflammatory messenger: Immunogenic exosome cargo in rheumatoid arthritis.

Our understanding of the role exosomes play in disease progression continues to develop and highlights their research potential to not only provide new insights into disease mechanisms, but their enormous diagnostic potential as well. Exosomes are small membrane encapsulated vesicles, typically 30-100nm in diameter and are actively secreted by most cell types studied. They contain DNA, RNA, protein and lipid cargo and have been observed to induce effects and changes in target cells when taken up.

Whilst the exosomes have in particular been studied in the context of cancer progression and biomarkers, recently it has emerged that during autoinflammatory conditions, they can potentially play a pathogenic role in disease progression.

Here, Foers et al use IonOpticks Aurora Series columns to comprehensively characterise synovial fluid (SF) exosomes which demonstrated their potential contributions to inflammatory pathways in Rheumatoid Arthritis. This work has led to a greater understanding of the immunomodulatory properties of synovial exosomes and how they may modulate the course of disease.

Read the full paper
Proteomic analysis of extracellular vesicles reveals an immunogenic cargo in rheumatoid arthritis synovial fluid.
Clinical & Translational Immunology. 2020 07 Nov. doi: https://doi.org/10.1002/cti2.1185
Foers AD, Dagley LF, Chatfield S, Webb AI, Cheng L, Hill AF, Wicks IP, Pang KC

Commentary by Andrew Webb, PhD.

About the author
Andrew has over 15 years’ experience in the field of chromatography and mass spectrometry. He is the lead innovator and inventor at IonOpticks, working closely with the team to test, refine and develop cutting edge techniques to support higher quality outputs and analytics from MS instruments. Andrew is also the Lab Head of the Walter and Eliza Hall Institute of Medical Research’s Proteomics Research Laboratory.