A new study proposes a strategy for analyzing newly synthesized proteins by integrating two different proteomic approaches, tandem orthogonal proteolysis (TOP) and cleavable bioorthogonal tagging (CBOT). This strategy (TOP-CBOT) allows for the detection and quantification of newly synthesized proteins in complex proteomes with high sensitivity and specificity. The authors tested the effectiveness of the strategy using both synthetic and biological samples and found that it provided accurate and reliable results. The study suggests that this technique can be a powerful tool for the study of the degradation kinetics of newly synthesized proteins and profiling of low abundant biomolecules. The strategy has the potential to contribute to the understanding of protein function and regulation in health and disease.
P. Meng; Y. Shao; Y. Xiong; L. Zhang; H. Bao; H. Lu
Peptide-and Protein-Level Combined Strategy for Analyzing Newly Synthesized Proteins by Integrating Tandem Orthogonal Proteolysis with Cleavable Bioorthogonal Tagging