A recent study using IonOpticks Aurora Series Columns proposes midiaPASEF, a method that improves the performance of data-independent acquisition proteomics. By analyzing peptide ions in a rapid serial manner, midiaPASEF increases the number of identified peptides and proteins with high accuracy. The results demonstrate that midiaPASEF outperforms previous approaches in terms of the number of peptides and proteins detected, making it an efficient and reliable method for proteomic analysis.
Ute Distler, Mateusz Krzysztof Lacki, Michał Piotr Startek, David Teschner, Sven Brehmer, Jens Decker, Thilo Schild, Jonathan Krieger, Florian Krohs, Oliver Raether, Andreas Hildebrandt and Stefan Tenzer
midiaPASEF maximizes information content in data-independent acquisition proteomics