Jarrod Sandow, PhD

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

Necroptosis is a lytic form of programmed cell death that is dysregulated in multiple human pathologies. Originating as a host defence mechanism against invading pathogens, necroptosis is triggered by various …

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis. Read More »

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

RNA polymerase II (RNAPII)-driven transcription is a highly conserved process with distinct checkpoints, each controlled by dedicated transcriptional cyclin-dependent kinases (CDKs). CDK9 controls the pause-release checkpoint following transcription initiation. CDK …

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check! Read More »

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that ubiquitinate and regulate cell surface expression of major histocompatibility complexes I and II as well as T- and B-cell co-activation receptors, …

Cell surface proteomics reveals major new targets for regulation by MARCH proteins. Read More »

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

Haematopoietic stem cells (HSCs) are responsible for continuous renewal of blood and immune cells, capable of both self-renewal and differentiation into effector cells. The balance of these processes is critical …

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells. Read More »

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, so profiling tumour pTyr levels may reveal therapeutic insights critical to combating disease. However, …

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr. Read More »

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

Mass spectrometry-based proteomics has transformed the study of cell signalling, enabling global studies of dynamic protein phosphorylation. A widespread post-translational modification, phosphorylation regulates almost all cellular processes, with two-thirds of …

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material. Read More »

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

In recent years mass spectrometry-based proteomics workflows have become ever more powerful in analysing complex proteomes to great depth. Data-independent acquisition (DIA) approaches are particularly attractive due to their reproducibility …

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension. Read More »

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

The concept of tissues as heterogeneous populations of cell types with diverse phenotypes is now well recognised thanks to single-cell transcriptomics. Although individual cell specialisation often arises from protein-mediated physiology, …

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity. Read More »

Rapid sample analysis: Simplified high-throughput methods for deep proteome analysis on timsTOF Pro.

Rapid sample analysis: Simplified high-throughput methods for deep proteome analysis on timsTOF Pro.

Recent advances in mass spectrometry technology have seen remarkable increases in proteomic sequencing speed, while improvements to dynamic range have remained limited. An exemplar of this is the new timsTOF …

Rapid sample analysis: Simplified high-throughput methods for deep proteome analysis on timsTOF Pro. Read More »