Although current mass-spectrometry (MS) methods can separately achieve deep proteome coverage, low missing data, high throughput, or high sensitivity, simultaneous combination of these has remained challenging. Here Derks et al. …
Pharmacologic inhibitors of cyclin-dependent kinases 4 and 6 (CDK4/6) have well-defined tumour-intrinsic cytostatic mechanisms. They are an approved treatment for hormone receptor–positive breast cancer and currently under evaluation in numerous …
#HUPOReCONNECT is in full swing and it’s been a jam-packed program of fantastic presentations, posters and networking events. If you haven’t registered yet, head here to access the full program. …
Digital microfluidics (DMF) technology facilitates miniaturised, automated, multiplexed liquid handling in multiple bioanalytical applications. However, in LC-MS-based proteomics its use has been limited to qualitative analysis, where the compatibility with …
Necroptosis is a lytic form of programmed cell death that is dysregulated in multiple human pathologies. Originating as a host defence mechanism against invading pathogens, necroptosis is triggered by various …
RNA polymerase II (RNAPII)-driven transcription is a highly conserved process with distinct checkpoints, each controlled by dedicated transcriptional cyclin-dependent kinases (CDKs). CDK9 controls the pause-release checkpoint following transcription initiation. CDK …
MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that ubiquitinate and regulate cell surface expression of major histocompatibility complexes I and II as well as T- and B-cell co-activation receptors, …
Cell surface proteomics reveals major new targets for regulation by MARCH proteins. Read More »
Haematopoietic stem cells (HSCs) are responsible for continuous renewal of blood and immune cells, capable of both self-renewal and differentiation into effector cells. The balance of these processes is critical …
I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …
Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »
Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, so profiling tumour pTyr levels may reveal therapeutic insights critical to combating disease. However, …
Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr. Read More »
Mass spectrometry-based proteomics has transformed the study of cell signalling, enabling global studies of dynamic protein phosphorylation. A widespread post-translational modification, phosphorylation regulates almost all cellular processes, with two-thirds of …
In recent years mass spectrometry-based proteomics workflows have become ever more powerful in analysing complex proteomes to great depth. Data-independent acquisition (DIA) approaches are particularly attractive due to their reproducibility …
The concept of tissues as heterogeneous populations of cell types with diverse phenotypes is now well recognised thanks to single-cell transcriptomics. Although individual cell specialisation often arises from protein-mediated physiology, …
Recent advances in mass spectrometry technology have seen remarkable increases in proteomic sequencing speed, while improvements to dynamic range have remained limited. An exemplar of this is the new timsTOF …
