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Revolutionizing Immunology and Oncology: Introducing IMBAS-MS, the Next-Generation Tool for Rapid and Sensitive Plasma Immunopeptide Analysis

13 September 2023

Revolutionizing Immunology and Oncology: Introducing IMBAS-MS, the Next-Generation Tool for Rapid and Sensitive Plasma Immunopeptide Analysis

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The study presents a significant advancement in immunopeptidomics, a field that explores the peptides presented by the human leukocyte antigen (HLA) protein, crucial in immune surveillance and immunotherapeutic strategies. The …

Revolutionizing Immunology and Oncology: Introducing IMBAS-MS, the Next-Generation Tool for Rapid and Sensitive Plasma Immunopeptide Analysis Read More »

Thunder-DDA-PASEF for identifying HLA class-I presented SARS-CoV-2 spike protein epitopes

4 July 2023

Thunder-DDA-PASEF for identifying HLA class-I presented SARS-CoV-2 spike protein epitopes

Latest News, Publications, Resource / By

The article “Thunder-DDA-PASEF enables high-coverage immunopeptidomics and identifies HLA class-I presented SarsCov-2 spike protein epitopes” describes a new method for identifying immunopeptides, which are a set of peptides presented by …

Thunder-DDA-PASEF for identifying HLA class-I presented SARS-CoV-2 spike protein epitopes Read More »

A new method to improve the quality of single-cell proteomics data

14 June 2023

A new method to improve the quality of single-cell proteomics data

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The article “Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics” published in Nature Methods, discusses a new method to improve the quality of single-cell proteomics …

A new method to improve the quality of single-cell proteomics data Read More »

plexDIA: sensitive proteomics with simultaneous deep coverage, high throughput, low missing data.

25 November 2021

plexDIA: sensitive proteomics with simultaneous deep coverage, high throughput, low missing data.

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Although current mass-spectrometry (MS) methods can separately achieve deep proteome coverage, low missing data, high throughput, or high sensitivity, simultaneous combination of these has remained challenging. Here Derks et al. …

plexDIA: sensitive proteomics with simultaneous deep coverage, high throughput, low missing data. Read More »

CDK4/6 inhibitors: critical regulation of T-cell memory fate commitment in tumour microenvironment.

18 November 2021

CDK4/6 inhibitors: critical regulation of T-cell memory fate commitment in tumour microenvironment.

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Pharmacologic inhibitors of cyclin-dependent kinases 4 and 6 (CDK4/6) have well-defined tumour-intrinsic cytostatic mechanisms. They are an approved treatment for hormone receptor–positive breast cancer and currently under evaluation in numerous …

CDK4/6 inhibitors: critical regulation of T-cell memory fate commitment in tumour microenvironment. Read More »

MS3 for TMT, not me! TMTProC- improved MS2 accuracy and sensitivity using complementary ions.

10 October 2021

MS3 for TMT, not me! TMTProC- improved MS2 accuracy and sensitivity using complementary ions.

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Quantitative multiplexed proteomic techniques, such as TMT, are a powerful tool for analysing and quantifying proteins in a cell. These techniques rely on the quantification of reporter ions that are …

MS3 for TMT, not me! TMTProC- improved MS2 accuracy and sensitivity using complementary ions. Read More »

On chip: multiplexed low input TMT-labelled quantitative proteomics with digital microfluidics.

5 October 2021

On chip: multiplexed low input TMT-labelled quantitative proteomics with digital microfluidics.

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Digital microfluidics (DMF) technology facilitates miniaturised, automated, multiplexed liquid handling in multiple bioanalytical applications. However, in LC-MS-based proteomics its use has been limited to qualitative analysis, where the compatibility with …

On chip: multiplexed low input TMT-labelled quantitative proteomics with digital microfluidics. Read More »

Interactions between SARS-CoV-2 viral proteins and host proteins reveal mechanisms of infection

30 September 2021

Interactions between SARS-CoV-2 viral proteins and host proteins reveal mechanisms of infection

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The Covid-19 pandemic is an ongoing global pandemic that has created an immense burden on health and economic systems globally, with 100s of millions of infections and millions of deaths …

Interactions between SARS-CoV-2 viral proteins and host proteins reveal mechanisms of infection Read More »

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

23 August 2021

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

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Necroptosis is a lytic form of programmed cell death that is dysregulated in multiple human pathologies. Originating as a host defence mechanism against invading pathogens, necroptosis is triggered by various …

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis. Read More »

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

16 August 2021

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

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RNA polymerase II (RNAPII)-driven transcription is a highly conserved process with distinct checkpoints, each controlled by dedicated transcriptional cyclin-dependent kinases (CDKs). CDK9 controls the pause-release checkpoint following transcription initiation. CDK …

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check! Read More »

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

1 August 2021

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

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MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that ubiquitinate and regulate cell surface expression of major histocompatibility complexes I and II as well as T- and B-cell co-activation receptors, …

Cell surface proteomics reveals major new targets for regulation by MARCH proteins. Read More »

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

4 July 2021

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

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Haematopoietic stem cells (HSCs) are responsible for continuous renewal of blood and immune cells, capable of both self-renewal and differentiation into effector cells. The balance of these processes is critical …

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells. Read More »

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics.

28 June 2021

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics.

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Globally, SARS-CoV-2 coronavirus has infected over 100 million individuals, caused millions of deaths, and dramatically impacted everyday life. The coronavirus is an enveloped RNA virus whose membrane spike glycoprotein – …

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics. Read More »

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

22 June 2021

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

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I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

22 June 2021

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

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DNA typically forms a right-handed double helix under physiological conditions but can also form higher-order structures such as a G-quadruplex (G4), formed by guanine-rich nucleic acid sequences. About 10,000 of …

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation. Read More »

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

16 May 2021

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

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Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, so profiling tumour pTyr levels may reveal therapeutic insights critical to combating disease. However, …

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr. Read More »

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

12 May 2021

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

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Mass spectrometry-based proteomics has transformed the study of cell signalling, enabling global studies of dynamic protein phosphorylation. A widespread post-translational modification, phosphorylation regulates almost all cellular processes, with two-thirds of …

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material. Read More »