Although current mass-spectrometry (MS) methods can separately achieve deep proteome coverage, low missing data, high throughput, or high sensitivity, simultaneous combination of these has remained challenging. Here Derks et al. …
Pharmacologic inhibitors of cyclin-dependent kinases 4 and 6 (CDK4/6) have well-defined tumour-intrinsic cytostatic mechanisms. They are an approved treatment for hormone receptor–positive breast cancer and currently under evaluation in numerous …
#HUPOReCONNECT is in full swing and it’s been a jam-packed program of fantastic presentations, posters and networking events. If you haven’t registered yet, head here to access the full program. …
Quantitative multiplexed proteomic techniques, such as TMT, are a powerful tool for analysing and quantifying proteins in a cell. These techniques rely on the quantification of reporter ions that are …
Digital microfluidics (DMF) technology facilitates miniaturised, automated, multiplexed liquid handling in multiple bioanalytical applications. However, in LC-MS-based proteomics its use has been limited to qualitative analysis, where the compatibility with …
The Covid-19 pandemic is an ongoing global pandemic that has created an immense burden on health and economic systems globally, with 100s of millions of infections and millions of deaths …
Aurora Series packed emitter columns are proudly setting a new standard of performance for leading mass specs. Having set an all new benchmark for what is possible, the Aurora Series …
Unlock the full potential of your mass spec with IonOpticks UHPLC columns Read More »
Necroptosis is a lytic form of programmed cell death that is dysregulated in multiple human pathologies. Originating as a host defence mechanism against invading pathogens, necroptosis is triggered by various …
RNA polymerase II (RNAPII)-driven transcription is a highly conserved process with distinct checkpoints, each controlled by dedicated transcriptional cyclin-dependent kinases (CDKs). CDK9 controls the pause-release checkpoint following transcription initiation. CDK …
MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that ubiquitinate and regulate cell surface expression of major histocompatibility complexes I and II as well as T- and B-cell co-activation receptors, …
Cell surface proteomics reveals major new targets for regulation by MARCH proteins. Read More »
Apoptosis is a highly regulated process of programmed cell death that occurs in multicellular organisms to eliminate unwanted and/or damaged cells. Apoptosis is therefore crucial for the maintenance of healthy …
Driving apoptosis; MIB2, an E3 ligase protein to the cockpit! Read More »
Haematopoietic stem cells (HSCs) are responsible for continuous renewal of blood and immune cells, capable of both self-renewal and differentiation into effector cells. The balance of these processes is critical …
Globally, SARS-CoV-2 coronavirus has infected over 100 million individuals, caused millions of deaths, and dramatically impacted everyday life. The coronavirus is an enveloped RNA virus whose membrane spike glycoprotein – …
I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …
Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »
DNA typically forms a right-handed double helix under physiological conditions but can also form higher-order structures such as a G-quadruplex (G4), formed by guanine-rich nucleic acid sequences. About 10,000 of …
Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, so profiling tumour pTyr levels may reveal therapeutic insights critical to combating disease. However, …
Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr. Read More »
Mass spectrometry-based proteomics has transformed the study of cell signalling, enabling global studies of dynamic protein phosphorylation. A widespread post-translational modification, phosphorylation regulates almost all cellular processes, with two-thirds of …
Mitochondria import almost their entire proteome from the cytoplasm by translocating precursor proteins through the translocase of the outer membrane (TOM) complex, embedded in the mitochondrial surface. The mitochondrial import …
The humoral antibody immune response is universal and critical for the survival of mammals, including humans. However, there is a lack of understanding of the antibody immune response mechanisms. Studying …
Pulmonary alveolar proteinosis (PAP) is a disorder causing accumulation of surfactant material in the lung. The autoimmune variant (aPAP) predominates, thought to be caused by elevated levels of granulocyte-macrophage colony-stimulating …
In recent years mass spectrometry-based proteomics workflows have become ever more powerful in analysing complex proteomes to great depth. Data-independent acquisition (DIA) approaches are particularly attractive due to their reproducibility …
