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‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis.

Necroptosis is a lytic form of programmed cell death that is dysregulated in multiple human pathologies. Originating as a host defence mechanism against invading pathogens, necroptosis is triggered by various …

‘Til death do us part: conformational change for MLKL, disengagement from RIPK3 in necroptosis. Read More »

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check!

RNA polymerase II (RNAPII)-driven transcription is a highly conserved process with distinct checkpoints, each controlled by dedicated transcriptional cyclin-dependent kinases (CDKs). CDK9 controls the pause-release checkpoint following transcription initiation. CDK …

PP2A, CDK9 transcription checkpoint interplay? Check! Targetable tumorigenesis inhibitor? Check! Read More »

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

Cell surface proteomics reveals major new targets for regulation by MARCH proteins.

MARCH proteins are membrane-associated Ring-CH E3 ubiquitin ligases that ubiquitinate and regulate cell surface expression of major histocompatibility complexes I and II as well as T- and B-cell co-activation receptors, …

Cell surface proteomics reveals major new targets for regulation by MARCH proteins. Read More »

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells.

Haematopoietic stem cells (HSCs) are responsible for continuous renewal of blood and immune cells, capable of both self-renewal and differentiation into effector cells. The balance of these processes is critical …

The truth about aging; proteomic characterisation of young and old mouse hematopoietic stem and progenitor cells. Read More »

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics.

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics.

Globally, SARS-CoV-2 coronavirus has infected over 100 million individuals, caused millions of deaths, and dramatically impacted everyday life. The coronavirus is an enveloped RNA virus whose membrane spike glycoprotein – …

Caution! Distinct glycosylation of SARS-CoV-2 RBD shed new light in its binding characteristics. Read More »

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

DNA typically forms a right-handed double helix under physiological conditions but can also form higher-order structures such as a G-quadruplex (G4), formed by guanine-rich nucleic acid sequences. About 10,000 of …

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation. Read More »

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr.

Tyrosine phosphorylation (pTyr) plays a pivotal role in signal transduction and is commonly dysregulated in cancer, so profiling tumour pTyr levels may reveal therapeutic insights critical to combating disease. However, …

Targeted tyrosine phosphorylation profiling in precision oncology using SureQuant pTyr. Read More »

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material.

Mass spectrometry-based proteomics has transformed the study of cell signalling, enabling global studies of dynamic protein phosphorylation. A widespread post-translational modification, phosphorylation regulates almost all cellular processes, with two-thirds of …

Phosphoproteomics with EasyPhos V2: Shorter workflow. Better sensitivity on limited starting material. Read More »

Club bouncer: the ubiquitin system dynamically regulates mitochondrial import at the TOM complex.

Club bouncer: the ubiquitin system dynamically regulates mitochondrial import at the TOM complex.

Mitochondria import almost their entire proteome from the cytoplasm by translocating precursor proteins through the translocase of the outer membrane (TOM) complex, embedded in the mitochondrial surface. The mitochondrial import …

Club bouncer: the ubiquitin system dynamically regulates mitochondrial import at the TOM complex. Read More »

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes.

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes.

The humoral antibody immune response is universal and critical for the survival of mammals, including humans. However, there is a lack of understanding of the antibody immune response mechanisms. Studying …

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes. Read More »

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity.

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity.

Pulmonary alveolar proteinosis (PAP) is a disorder causing accumulation of surfactant material in the lung. The autoimmune variant (aPAP) predominates, thought to be caused by elevated levels of granulocyte-macrophage colony-stimulating …

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity. Read More »

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

In recent years mass spectrometry-based proteomics workflows have become ever more powerful in analysing complex proteomes to great depth. Data-independent acquisition (DIA) approaches are particularly attractive due to their reproducibility …

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension. Read More »

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

The concept of tissues as heterogeneous populations of cell types with diverse phenotypes is now well recognised thanks to single-cell transcriptomics. Although individual cell specialisation often arises from protein-mediated physiology, …

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity. Read More »

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target.

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target.

Cytotoxic T lymphocytes (CTLs) are a specialised form of killer cells which are capable of moving around the body, seeking out harmful cells, and removing them from the body before …

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target. Read More »

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1.

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1.

Mutations in FAS play a crucial role in the development of severe immunodeficiency, which is promoted by aberrant T lymphocyte death. In addition to this, mutations in caspase-8 or FADD, …

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1. Read More »

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding.

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding.

Apart from 13 core subunits of the OXPHOS pathway, all the human mitochondrial proteome is encoded by the nuclear genome. Following synthesis on cytosolic ribosomes, proteins for mitochondrial function are …

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding. Read More »

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code.

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code.

Targeted protein degradation has recently been studied as a possible therapeutic paradigm. Kaiho-Soma et al. were able to establish that the ubiquitin ligase thyroid hormone receptor-interacting protein 12 (TRIP12) accelerates …

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code. Read More »

The secret interface of the neuroimmune system: the key to immune surveillance.

The secret interface of the neuroimmune system: the key to immune surveillance.

Neuroimmune interactions play a significant role in several neurological disorders and the current state-of-art suggests that central nervous system (CNS)-derived antigens are sorted and presented to circulating T cells. However, …

The secret interface of the neuroimmune system: the key to immune surveillance. Read More »