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Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov

I was fortunate to sit down with Associate Professor Nikolai Slavov to chat about the SCoPE2 method, an improvement on the previously developed SCoPE-MS method, using multiplexed LC-ESI-MS/MS for single-cell …

Single-Cell Proteomics and the SCoPE2 method with Associate Professor Nikolai Slavov Read More »

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation.

DNA typically forms a right-handed double helix under physiological conditions but can also form higher-order structures such as a G-quadruplex (G4), formed by guanine-rich nucleic acid sequences. About 10,000 of …

G4 nucleic acid structures act as extra-chromosomal inhibitors in ISG cancer gene regulation. Read More »

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes.

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes.

The humoral antibody immune response is universal and critical for the survival of mammals, including humans. However, there is a lack of understanding of the antibody immune response mechanisms. Studying …

Big data and structural proteomics allow epitope mapping of over 100,000 antigen-nanobody complexes. Read More »

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity.

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity.

Pulmonary alveolar proteinosis (PAP) is a disorder causing accumulation of surfactant material in the lung. The autoimmune variant (aPAP) predominates, thought to be caused by elevated levels of granulocyte-macrophage colony-stimulating …

Hitching timsTOF to the proteogenomics wagon takes autoantibody clonal analysis to the next level of sensitivity. Read More »

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension.

In recent years mass spectrometry-based proteomics workflows have become ever more powerful in analysing complex proteomes to great depth. Data-independent acquisition (DIA) approaches are particularly attractive due to their reproducibility …

diaPASEF: taking deep analysis of complex proteomes with DIA workflows into a new di(a)mension. Read More »

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity.

The concept of tissues as heterogeneous populations of cell types with diverse phenotypes is now well recognised thanks to single-cell transcriptomics. Although individual cell specialisation often arises from protein-mediated physiology, …

SCoPE this out! Improved single-cell proteomics method exposes macrophage proteome heterogeneity. Read More »

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target.

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target.

Cytotoxic T lymphocytes (CTLs) are a specialised form of killer cells which are capable of moving around the body, seeking out harmful cells, and removing them from the body before …

Killers that communicate: Cytotoxic T lymphocytes are capable of swarming to their tumour target. Read More »

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1.

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1.

Mutations in FAS play a crucial role in the development of severe immunodeficiency, which is promoted by aberrant T lymphocyte death. In addition to this, mutations in caspase-8 or FADD, …

The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1. Read More »

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding.

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding.

Apart from 13 core subunits of the OXPHOS pathway, all the human mitochondrial proteome is encoded by the nuclear genome. Following synthesis on cytosolic ribosomes, proteins for mitochondrial function are …

Getting their ducks in a row – LONP1 co-chaperones with mtHSP70 in mitochondrial protein folding. Read More »

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code.

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code.

Targeted protein degradation has recently been studied as a possible therapeutic paradigm. Kaiho-Soma et al. were able to establish that the ubiquitin ligase thyroid hormone receptor-interacting protein 12 (TRIP12) accelerates …

Ubiquitin ligase marks neo-substrates for degradation with unique Ub code. Read More »

The secret interface of the neuroimmune system: the key to immune surveillance.

The secret interface of the neuroimmune system: the key to immune surveillance.

Neuroimmune interactions play a significant role in several neurological disorders and the current state-of-art suggests that central nervous system (CNS)-derived antigens are sorted and presented to circulating T cells. However, …

The secret interface of the neuroimmune system: the key to immune surveillance. Read More »

Recombinant lectins as novel and efficient glycoproteomic tools.

Recombinant lectins as novel and efficient glycoproteomic tools.

Post-translational modifications (PTMs) are crucial biological processes implicated in multiple physiological and pathological pathways. Glycosylation is one such PTM that results in the attachment of glycans, which are chain-like structures …

Recombinant lectins as novel and efficient glycoproteomic tools. Read More »

Getting to the heart of the matter: Comprehensive peptide & oxidation profiling in failing human hearts with UHPLC-MS.

Getting to the heart of the matter: Comprehensive peptide & oxidation profiling in failing human hearts with UHPLC-MS.

Diabetes, obesity, heart disease and ageing have all been strongly associated with oxidative stress. For heart disease in particular, the resultant oxidative protein damage caused has been directly linked to …

Getting to the heart of the matter: Comprehensive peptide & oxidation profiling in failing human hearts with UHPLC-MS. Read More »

Peroxisomal retrograde signalling in stress; an alternate mechanistic dimension.

Peroxisomal retrograde signalling in stress; an alternate mechanistic dimension.

Peroxisomes are ubiquitous to all eukaryotes and play a vital role in growth, development as well as stress response in single- and multicellular microorganisms, plants and animals. Disorders attributed to …

Peroxisomal retrograde signalling in stress; an alternate mechanistic dimension. Read More »

Caught in the act: New CryoEM protein structure reveals the potential to drug antibiotic resistance.

Caught in the act: New CryoEM protein structure reveals the potential to drug antibiotic resistance.

How bacteria resistance can be spread between species of bacteria is understood to occur via a couple mechanisms. One of these such mechanisms involves cell surface proteins that can bind …

Caught in the act: New CryoEM protein structure reveals the potential to drug antibiotic resistance. Read More »

diaPASEF on the Bruker timsTOF Pro + Aurora Series columns provides unprecedented proteome analysis.

diaPASEF on the Bruker timsTOF Pro + Aurora Series columns provides unprecedented proteome analysis.

Over the last few years, mass spectrometry based proteomics workflows have become increasingly more powerful in capturing complex proteomes to incredible depths. This has largely been driven by the adoption …

diaPASEF on the Bruker timsTOF Pro + Aurora Series columns provides unprecedented proteome analysis. Read More »

An inflammatory messenger: Immunogenic exosome cargo in rheumatoid arthritis.

An inflammatory messenger: Immunogenic exosome cargo in rheumatoid arthritis.

Our understanding of the role exosomes play in disease progression continues to develop and highlights their research potential to not only provide new insights into disease mechanisms, but their enormous …

An inflammatory messenger: Immunogenic exosome cargo in rheumatoid arthritis. Read More »