The concept of tissues as heterogeneous populations of cell types with diverse phenotypes is now well recognised thanks to single-cell transcriptomics. Although individual cell specialisation often arises from protein-mediated physiology, …
Cytotoxic T lymphocytes (CTLs) are a specialised form of killer cells which are capable of moving around the body, seeking out harmful cells, and removing them from the body before …
Mutations in FAS play a crucial role in the development of severe immunodeficiency, which is promoted by aberrant T lymphocyte death. In addition to this, mutations in caspase-8 or FADD, …
The FAScinating truth: Innate immune signalling driven by caspase-8 cleavage of N4BP1. Read More »
Apart from 13 core subunits of the OXPHOS pathway, all the human mitochondrial proteome is encoded by the nuclear genome. Following synthesis on cytosolic ribosomes, proteins for mitochondrial function are …
Targeted protein degradation has recently been studied as a possible therapeutic paradigm. Kaiho-Soma et al. were able to establish that the ubiquitin ligase thyroid hormone receptor-interacting protein 12 (TRIP12) accelerates …
Ubiquitin ligase marks neo-substrates for degradation with unique Ub code. Read More »
Neuroimmune interactions play a significant role in several neurological disorders and the current state-of-art suggests that central nervous system (CNS)-derived antigens are sorted and presented to circulating T cells. However, …
The secret interface of the neuroimmune system: the key to immune surveillance. Read More »
The interactome of membrane proteins are particularly interesting in cancer research as their involvement in pathways provide possible candidates for drug targets, as well as, understanding the molecular mechanisms involved …
Tumor suppressor PRELP membrane targets finally identified. Read More »
Plasma is a powerful reservoir of disease biomarkers for diagnostic, prognostic, and predictive purposes, flowing through each tissue in the body and accumulating vital information about the pathological state of …
Getting into the rhythm with large-scale profiling of the plasma proteome. Read More »
Post-translational modifications (PTMs) are crucial biological processes implicated in multiple physiological and pathological pathways. Glycosylation is one such PTM that results in the attachment of glycans, which are chain-like structures …
Recombinant lectins as novel and efficient glycoproteomic tools. Read More »
Diabetes, obesity, heart disease and ageing have all been strongly associated with oxidative stress. For heart disease in particular, the resultant oxidative protein damage caused has been directly linked to …
Peroxisomes are ubiquitous to all eukaryotes and play a vital role in growth, development as well as stress response in single- and multicellular microorganisms, plants and animals. Disorders attributed to …
Peroxisomal retrograde signalling in stress; an alternate mechanistic dimension. Read More »
How bacteria resistance can be spread between species of bacteria is understood to occur via a couple mechanisms. One of these such mechanisms involves cell surface proteins that can bind …
Over the last few years, mass spectrometry based proteomics workflows have become increasingly more powerful in capturing complex proteomes to incredible depths. This has largely been driven by the adoption …
Our understanding of the role exosomes play in disease progression continues to develop and highlights their research potential to not only provide new insights into disease mechanisms, but their enormous …
An inflammatory messenger: Immunogenic exosome cargo in rheumatoid arthritis. Read More »
Dr Jarrod Sandow provides an overview of the beginnings of IonOpticks and our Aurora Series columns, currently the global leader in high-performance packed emitter columns.
The importance of exosomes in many facets of biology continues to grow, having been implicated in many fundamental processes of cellular and host function. Exosomes are small membrane encapsulated vesicles, …
Delivery with a killer message: Airway exosomes drive antiviral response. Read More »
Tumour Necrosis Factor (TNF) plays a central role in our body’s immune system, helping to control inflammation that occurs as our body responds to damage or infection. Cells often respond …
Releasing the brakes on immune responses by inhibition of HOIL-1L. Read More »
RNA Polymerase is a multi-subunit enzyme that reads DNA and generates messenger RNA in the process known as transcription. Transcription, as the first step in gene expression, is tightly regulated …
Deep label free phosphoproteomics identifies novel mechanisms of kinase function. Read More »
Growth factors and their receptors are central in directing cell division, development and differentiation. Their role in controlling cell fate makes them one of the most hijacked protein classes during …
The tumor microenvironment, made up of surrounding fibroblasts, immune cells and blood vessels, is much less studied and understood than the biology of the cancerous tissue itself. It is well …
Label free secretome analysis demonstrates microenvironment regulation of cancer. Read More »
Phosphotyrosine (pTyr) plays a central role in many cell-to-cell communication pathways, including those that regulate proliferation, differentiation, adhesion, hormone responses, and immune defense. These pathways are commonly dysregulated in cancer …
A new sensitive and accurate method enables endogenous phosphotyrosine mapping. Read More »
