May 13
@
7:00 pm
–
8:00 pm
Hosted by Sales Director, Zac Anderson, this live IonOpticks Insights webinar joins Dr Brendon Seale as he explores the role of commercially prepared nano flow chromatography columns in modern proteomics core facilities.
WEDNESDAY, MAY 13
Commercial advantage: reproducibility and speed of commercially prepared nano flow chromatography columns for the proteomics core lab
Join LTRI’s Proteomics Core Scientific Manager Dr Brendon Seale for our next IonOpticks Insights webinar: ‘Reproducibility and speed of commercially prepared nano flow chromatography columns for the proteomics core lab.’
Running a high-throughput core facility means serving diverse users, juggling multiple instrument setups, and keeping analytical performance consistently high. This webinar explores how commercially prepared nano flow columns are meeting that challenge head-on.
Drawing on hands-on experience at the Lunenfeld-Tanenbaum Research Institute, Dr Seale will cover how Aurora Series® columns deliver the reproducibility and peak capacity needed to scale to hundreds of samples per day, without sacrificing depth of coverage, and how integrated column heating helps maintain stability across varied workflows and users.
He’ll also speak to the operational case for moving away from in-house column packing, and what that shift unlocks for core labs focused on high-value outputs.
Abstract
Commercial advantage: Reproducibility and speed of commercially prepared nanoflow chromatography columns for the proteomics core labLiquid chromatography (LC) is often forgotten in the discussion of mass spectrometry-based proteomics. Generally, the sensitivity and resolution of mass spectrometers receive far more attention. But in many cases LC is the component responsible for the reproducibility and robustness necessary for quality data, especially in the context of core labs. At the inception of low-flow chromatography for bottom-up proteomics, a ‘DIY’ attitude was definitely the norm. But the changing landscape of the industry has brought more commercially produced columns onto the market. In this presentation, we explore the benefits of commercial columns for micro/nano-flow chromatography in bottom-up proteomics. These columns not only provide performance that is difficult to achieve reliably with a self-packed system, but are also much more amenable to the fast, sensitive instruments of today. The use of these technologies is rapidly increasing the throughput of the lab, not just to tens of samples per day, but hundreds, with excellent performance and reproducibility which would have been unheard of only a matter of years ago.
About the presenter
Dr Brendon SealeDr Brendon Seale holds a PhD in Bioanalytical Chemistry from the University of Toronto, where he developed approaches to reducing sample complexity using automated microfluidics and differential mobility spectrometry coupled with mass spectrometry. He went on to complete a Mitacs ELEVATE postdoctoral fellowship with Derek Wilson (York University & SCIEX), developing ion mobility methods for peptide and protein analysis in the gas phase.
He subsequently joined the lab of Anne-Claude Gingras at the LTRI, working on miniaturisation of proximity protein interaction methods before being appointed Scientific Manager of the Proteomics Core facility of the Network Biology Collaborative Centre. In this role, he leads the development and implementation of advanced technologies for sample preparation and mass spectrometry, and oversees the training of graduate students in mass spectrometry.
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