Generation 4 Aurora Rapid® 8×75 nano flow columns are purpose-built for high-throughput LC-MS workflows, delivering unmatched performance for the analysis of single cells and low-input proteomics. Designed with an ultra-narrow internal diameter and a compact 8 cm bed length, these columns enable exceptional sensitivity and separation efficiency, even in the shortest gradient times.

Whether you’re processing thousands of samples in a large-scale study or working with trace-level biological material, Aurora Rapid® 8×75 delivers unparalleled depth of proteome coverage without sacrificing data quality. The columns are optimised for ultra-fast gradients, reducing run times while maintaining precise peak shapes and reproducible retention times, crucial for confident identification and quantification in demanding experimental designs.

Ideal for laboratories prioritising speed, precision, and sensitivity, the Gen 4 Aurora Rapid® 8×75 columns set a new benchmark for throughput and performance in cutting-edge single-cell and low-sample-load proteomics.

Product Benefits

• High throughput
• Sensitivity
• Analyse more cells in less time

HeatSync™ highlights

• Simple plug and play format
• Intuitive design, removes unnecessary complexity
• Single button, dual control
• Rotary touch interface
• Unique heater design for seamless integration
• Reusable and universally compliant

Incredibly stable retention times

Retention times were highly consistent across multiple columns and injections, with minimal drift observed – this level of chromatographic reproducibility is critical for large-scale and longitudinal studies.

Stable peptide retention times across different columns. Ten peptides were selected and their retention times assessed across all columns from 3 HeLa tryptic digest injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using an 80 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analyzed in Spectronaut version 19.8 with the “Match between runs” feature disabled.

Column backpressure stability and protein group IDs over extended use

The figure below shows backpressure at 8 minutes over more than 650 QC runs using 60 SPD method, with only a gradual increase from 130 to 175 bar and consistently high unique protein group IDs. Complex samples were analysed between QC runs, highlighting column’s robustness and suitability for high-throughput proteomics.

Backpressure stability and protein group IDs over extended column use from HeLa tryptic digest QC injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using a 60 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analysed in Spectronaut version 19.8 with the “Match between runs” feature disabled.