A dilution series of a HeLa tryptic digest was separated on an Aurora Rapid75™ column using an Evosep One and Bruker timsTOF SCP (dia-PASEF) using an 80 samples per day method (currently under development), n = 6. Data courtesy of Dr Christoph Krisp (Bruker), Dorte Bekker-Jensen and Dr Nicolai Bache (Evosep).

A HeLa tryptic digest (1 ng) was separated on an Aurora Rapid75™ column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nL/min. The extracted ion chromatograms for 7 selected peptides from the analysis are shown. The average Full Width at Half Maximum (FWHM, seconds) for each peptide across 30 runs is shown above each peak.