25 cm x 75 μm C18 UHPLC packed emitter column with built-in HeatSync™ technology, compatible with EASY-Spray, Nanospray Flex, and Newomics UniESI and DuoESI ion sources.
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Product Information
The original game-changer.
The pinnacle of chromatography performance while minimizing overall cost and stress on equipment. Want it all? The Aurora Ultimate™ continues the legacy of IonOpticks’ original 25 cm UHPLC line, delivering maximum peak capacity when time is less of a determining factor, but still a consideration.
Product Benefits:
Extreme coverage and sensitivity without excessive time
Unlocks the true power of your mass spectrometer
Spectacular protein IDs. Every run. Every time.
Deep proteome coverage.
Maximise the number of identifications that you can achieve from a single shot sample analysis.
Figures 1, 2 & 3: Identified unique proteins, peptides and average Full Width at Half Maximum (FWHM) from 200 ng HeLa Tryptic Digest injection on an Aurora Ultimate™ 25 cm x 75 μm column using different gradient lengths. Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.
Figure 4: Yeast tryptic digest was spiked into a HeLa tryptic digest (200 ng) in different ratios (Sample A – 45 ng; Sample B – 15 ng). Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition using an Aurora 25 cm x 75 μm column. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples. Figure 5: Boxplot demonstrating average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.
High quantitative accuracy
Identify large numbers of unique proteins without compromising quantitative accuracy.
Dr. Alejandro Brenes, a Wellcome Trust Early Career Fellow at the Universit...
Compatible with EASY-Spray
Compatible with Newomics Duo/UniESI
Compatible with Nanospray Flex
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