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HUPO 2023
17 - 21 September 2023, Busan, South Korea
We're so excited to be attending the 22nd HUPO Annual World Congress, taking place 17 - 21 September 2023 in Busan, South Korea.
Learn more about our products and catch up with our team while there. We can't wait to see you in Asia for a second time this year!
Where to find us
Booth 210
Poster Presentations by IonOpticks
| Title: Unlocking the full potential of the timsTOF platform using packed emitter columns Authors; Jarrod J Sandow, Jenna Cowen, Yanxiang Meng Date and Time: September 19, 2023 3.35 PM - 4:30 PM Location: Exhibit Hall Abstract: The timsTOF mass spectrometer has revolutionized proteomics research with its exceptional capabilities in speed and sensitivity. However, the full potential of this platform can only be realised by partnering this technology with high-performance chromatography and peptide separation. Aurora Series packed emitter columns are designed to optimize peptide separations and ion transmission efficiency by eliminating dead volumes from the analytical column, ultimately leading to dramatically improved sensitivity, dynamic range, and robustness. By incorporating these columns into the timsTOF workflow we were able to significantly enhance the depth and quality of proteome coverage.Aurora Series columns offered superior resolving power compared to other commercially available columns, allowing for enhanced separation of complex peptide mixtures. This resulted in increased identifications and quantification of peptides and proteins with more than 6500 proteins identified in 30 mins. The high ion transmission efficiency of Aurora Series columns led to improved signal intensity and sensitivity. This was particularly advantageous for single-cell applications with more than 3000 proteins identified from true single-cell samples. Moreover, the compatibility of Aurora Series columns with the timsTOF system ensures seamless integration and optimal performance. The combination of Aurora Series columns and timsTOF technology allows for robust and reproducible proteomic analyses, allowing scientists to discover more proteins from a large variety of research samples.The utilisation of IonOpticks’ Aurora Series columns on the timsTOF platform offers substantial advantages for proteomics research. Researchers can expect enhanced peptide separations, increased sensitivity, and improved robustness, leading to deeper insights into complex biological systems. These advancements in performance will undoubtedly accelerate discoveries and drive breakthroughs in the field of proteomics. READ MORE > |
| Title: Approaching single-shot, full proteome coverage using packed emitter columns Authors; Yanxiang Meng, Jarrod J Sandow, Jenna Cowen Date and Time: September 19, 2023 3.35 PM - 4:30 PM Location: Exhibit Hall Abstract: Full proteome analysis is a long-standing aspirational goal in the field of proteomics. The advent of high-resolution mass spectrometry, coupled with advancements in chromatographic separation, is bringing us closer to achieving this objective. IonOpticks’ Aurora Series packed emitter columns, when combined with Thermo Scientific’s Orbitrap mass spectrometers, provide a powerful platform for achieving deep and comprehensive proteomic profiling. When used to analyze complex proteome samples, the IonOpticks Frontier column (60 cm x 75 μm i.d.), specifically optimized for deep proteome analysis, exhibited exceptional resolving power and robustness. It enabled highly efficient chromatographic separations, resulting in improved peak capacity and enhanced identification and quantification of proteomic samples.The integration of IonOpticks’ Aurora Frontier column with Thermo Scientific’s Orbitrap mass spectrometers allowed the identification of greater than 10,000 proteins from a single-shot - which is approaching full proteome coverage. This achievement was attributed to the unique features of Aurora Series columns, including their elimination of dead volumes and unique fittings capable of withstanding >1700bar. These attributes contributed to increased separation efficiency, reduced peak broadening, and enhanced detection sensitivity, ultimately enabling the identification of a greater number of peptides and proteins in a single-shot analysis. Moreover, the robustness and reproducibility of IonOpticks’ Aurora Series columns on Thermo Scientific’s mass spectrometers ensured consistent results across multiple experimental runs.The implementation of IonOpticks’ Aurora Series packed emitter columns on Thermo Scientific’s Orbitrap mass spectrometers represents a significant breakthrough in proteomic research. Achieving single-shot full proteome coverage will open new avenues for in-depth exploration of complex biological systems. READ MORE > |
| Title: Utilization of the speed, sensitivity and accuracy of the ZenoTOF 7600 to enhance protein identifications from packed emitter columns Authors; Jenna Cowen, Yanxiang Meng, Jarrod J Sandow Date and Time: September 19, 2023 3.35 PM - 4:30 PM Location: Exhibit Hall Abstract: The field of proteomics demands high-performance mass spectrometry tools to achieve comprehensive protein identifications, sensitive analysis, and reliable quantitation. IonOpticks’ Aurora Series SX packed emitter columns have been specifically designed to optimize peptide and protein analysis on the ZenoTOF 7600 mass spectrometer, enabling researchers to unlock a new level of sensitivity. This enhanced sensitivity allows for the identification and quantification of proteins from complex samples with unprecedented accuracy, significantly expanding the depth of proteome coverage. Researchers can now confidently explore the complex proteomic landscape and uncover novel biomarkers or protein interactions that were previously challenging to identify.Furthermore, the combination of IonOpticks’ Aurora Series SX columns and the ZenoTOF 7600 mass spectrometer ensures stable quantitation across multiple samples. The Aurora Series SX columns exhibited excellent reproducibility, providing consistent results and minimizing variability in quantitative analyses. The high-sensitivity analysis facilitated by the Aurora Series SX columns on the ZenoTOF 7600 mass spectrometer, coupled with stable quantitation, represents a powerful approach for comprehensive proteomics research. We were able to confidently identify more than 7300 proteins per run on a 45 min gradient with a majority of proteins having a CV >10%. The ability to maximize protein identifications, accurately quantify protein expression, and maintain analytical reproducibility empowers scientists to unravel complex biological processes and gain deeper insights into disease mechanisms, drug responses, and cellular signaling pathways.In summary, the integration of IonOpticks’ Aurora Series READ MORE > |
Posters
Title: Exploring the potential of transplanted colon organoids to mimic human physiology in mice using Deep Visual Proteomics
Authors; Fredrick Post
Date and Time: September 18, 2023 11:45 AM - 1:00 PM
Location: Exhibit Hall
Abstract: Organoids are of great potential to study human biology and explore new therapeutic applications. With their 3D structure and ability to recapitulate physiological differentiation patterns, organoids provide valuable insights beyond traditional immortalized cell lines. Moreover, colon organoids can be successfully transplanted into mice (Watanabe et al., 2022), which enables the examination of epithelial cells in their in vivo context.
Methods: Stem cell-derived colon organoids in culture, in vivo, and healthy human colon samples were analyzed using the Deep Visual Proteomics (DVP) workflow (Mund et al., 2022), incl. markers for stem cells, epithelial cells, goblet cells, immune cells, and fibroblasts. Proteomes of the cell populations were acquired using the timsTOF SCP in py_diAID acquisition mode coupled to an Evosep running Whisper 40 gradient on a 15 cm Ionopticks Aurora ELITE column.
Results: By combining high-resolution imaging, single-cell image analysis, laser micro dissection, and mass spectrometry, we got unprecedented insights into organoid biology. More than 4000 proteins were identified across cell populations. We captured proteome dynamics of organoids from growing in culture, to transplantation and establishment within the microenvironment of the murine colon. The comparison of cell populations in healthy human colon crypts and transplanted organoids enabled us to assess to which extent organoids in vivo can recapitulate human colon tissue.
Conclusion: To further explore the potential of in vivo organoid studies, we adapted the DVP workflow for the analysis of experiments using organoids. By utilizing the capabilities of spatial and single-cell analysis of DVP, researchers can enhance organoid research, such as by uncovering the mode of action of therapeutics in organoid drug screens.
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Meet our team attending
