Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics

Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics

Dijana

2 August 2023
Publications / By

The article titled “Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics” describes a new data acquisition method for quantifying a large number of peptides in plasma using multiplexed targeted proteomics. Lesur et al. improved upon the parallel accumulation and serial fragmentation (prm-PASEF) technique, and developed a less time-consuming alternative known as guided data independent acquisition (dia)-PASEF (g-dia-PASEF). These methods were used to quantify 565 plasma proteins using 782 labelled peptides, and analyzed blood samples from a cohort of patients with colorectal cancer. This allowed the authors to identify 14 differentially regulated proteins in these patients comparing to the control samples, which demonstrated the potential of this method to provide a rapid and unbiased way to quantify a large number of peptides in plasma, making it a powerful tool for biomarker discovery and validation.

This study utilized our 25cm Aurora Ultimate column, increasing sensitivity and coverage.

Read the full paper here – Journal Article – 2023 – Journal of Proteome Research

Authors

A. Lesur; F. Bernardin; E. Koncina; E. Letellier; G. Kruppa; P.-O. Schmit; G. Dittmar

Title

Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics

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Byoung-Kyu Cho, Team Lead, Proteomics
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Washington University in St. Louis, School of Medicine

With over a decade of experience in proteomics research, I can confidently say that the IonOpticks column is the superior choice. Its exceptional analytical performance in terms of sensitivity and reproducibility has allowed me to maximize protein identification in any biological sample. Additionally, the column is robust and can withstand hundreds of runs without significant loss of performance. The customer service team is outstanding, always quick to respond to any questions or concerns. I highly recommend IonOpticks columns to any researcher seeking high-quality mass spectrometry data.

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The Aurora columns are fantastic. Not only do they perform better than other columns on the market but they're durable and the single nano connection makes setup easy and simplifies troubleshooting. I use them for all my routine peptide and PTM applications.

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We switched all our instruments with a 15 or 25cm aurora column for 1 year. Their performance, spray stability and robustness are really appreciated. We have really good results in label-free and DIA workflow compared to other columns used.

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Northeastern University

We obtained better results with IonOpticks...

 

We were able to leverage the very high accuracy of retention times as an additional feature to increase confidence in correct peptide identifications and to reduce confidence in incorrect ones.

Akinori Endo, PhD
Akinori Endo, PhD

Laboratory of Protein Metabolism
Tokyo Metropolitan Institute of Medical Science
Tokyo, Japan

We are very pleased with the performance of the Aurora column that can be applied to most proteomics studies. They allow us to acquire MS data with higher quality and especially improved in-depth proteomics output.

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Edinburgh Cancer Research UK Centre
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We have switched all our instruments from in-house packed 35cm emitters to Aurora columns. Their performance is equal or superior to our in-house packed columns, their spray-stability and durability is exceptional, they are fool-proof to install and last hundreds of runs.

Martin R. Larsen, PhD
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Protein Research Group, University of Southern Denmark
Odense, Denmark

We have recently started to use the Aurora columns and they significantly outperform any other columns that we have ever used for nano-LC-MSMS, by providing much higher peptide coverage in proteomics and phosphoprotemics.

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Ludwig-Maximilians-Universität
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