Oxygen toxicity causes cyclic damage by destabilizing specific Fe-S cluster-containing protein complexes

Oxygen toxicity causes cyclic damage by destabilizing specific Fe-S cluster-containing protein complexes

Dijana

16 August 2023
Publications / By

This publication investigates the mechanism of how oxygen toxicity, related to excessive oxygen exposure, damages living cells. Researchers found that high levels of oxygen disrupt specific protein complexes containing iron-sulfur (Fe-S) clusters, leading to oxidative damage and cell death. The study, which utilizes our Aurora Elite column, shows how these protein complexes are essential for the survival of organisms, and the disruption of these clusters by oxygen toxicity is a crucial factor in many diseases. Understanding this mechanism could aid in developing therapies to protect against oxygen toxicity-related damage. The study provides insight into the fundamental process of cellular respiration and highlights the need for maintaining proper oxygen levels to maintain healthy cellular function.

This study utilized our Aurora Ultimate column, increasing sensitivity and coverage.

Read the full paper here – Journal Article – 2023 – Molecular Cell

Authors

A. H. Baik; A. G. Haribowo; X. Chen; B. B. Queliconi; A. M. Barrios; A. Garg; M. Maishan; A. R. Campos; M. A. Matthay; I. H. Jain

Title

Oxygen toxicity causes cyclic damage by destabilizing specific Fe-S cluster-containing protein complexes

 

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Northeastern University

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We were able to leverage the very high accuracy of retention times as an additional feature to increase confidence in correct peptide identifications and to reduce confidence in incorrect ones.

Akinori Endo, PhD
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Laboratory of Protein Metabolism
Tokyo Metropolitan Institute of Medical Science
Tokyo, Japan

We are very pleased with the performance of the Aurora column that can be applied to most proteomics studies. They allow us to acquire MS data with higher quality and especially improved in-depth proteomics output.

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Edinburgh Cancer Research UK Centre
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Protein Research Group, University of Southern Denmark
Odense, Denmark

We have recently started to use the Aurora columns and they significantly outperform any other columns that we have ever used for nano-LC-MSMS, by providing much higher peptide coverage in proteomics and phosphoprotemics.

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